|
| Status |
Public on Aug 06, 2017 |
| Title |
control blastocyst 8 (no bisection) |
| Sample type |
RNA |
| |
|
| Source name |
blastocyst stage embryo
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: B6C3F1 x CD1
age: Theiler stage 5; 4 dpc
cell number: normal
tissue: blastocyst stage embryo
|
| Treatment protocol |
At the 2-cell stage embryos were split mechanically, and each blastomere was cultured individually in the well of a 96-well plate until the blastocyst stage, under 5% CO2 and atmospheric O2
|
| Growth protocol |
1-cell zygotes from B6C3F1 x CD1 mating (stimulated cycle) were collected from the oviducts and cultured in pools in KSOM(aa) medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Individual blastocysts were lysed in in Zymo buffer (Quick RNA micro prep) and total RNA was extracted in water according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared using GeneChip WT Termina Labeling kit, reverse-transcribing 20 micrograms cRNA and, of these, subjecting 5.5 micrograms to labeling
|
| |
|
| Hybridization protocol |
The GeneChip Hybridization, Wash and Stain Kit was used, for 16 h (+/- 1h) at 45°C with 60 rpm. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
The GeneChips were scanned using the Affymetrix 3000 7G scanner.
|
| Description |
Single embryo was lysed in Zymo buffer (Quick RNA micro prep) and total RNA was extracted in water
Sample name: ctrl8
|
| Data processing |
The robust multiarray averaging method was applied for background correction, normalization, and probe summarization using the Affymetrix Expression Console.
|
| |
|
| Submission date |
Nov 29, 2016 |
| Last update date |
Aug 06, 2017 |
| Contact name |
Michele Boiani |
| E-mail(s) |
[email protected]
|
| Organization name |
Max Planck Institute Muenster
|
| Street address |
Roentgenstrasse 20
|
| City |
Muenster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE90674 |
Single embryo analysis of monozygotic twin blastocysts of the mouse respecting pair associations |
|
