|
| Status |
Public on Feb 06, 2017 |
| Title |
PS4 |
| Sample type |
RNA |
| |
|
| Source name |
Stripped oocytes from an individual scallop
|
| Organism |
Pecten maximus |
| Characteristics |
sample type: gamete stripping
|
| Growth protocol |
Pecten maximus (mean weight±SD: 174±32g, mean length: 111±7mm) were caught from the bay of Brest at the beginning of their natural spawning period and transferred to the experimental hatchery of Ifremer (Argenton, France) where they were conditioned for 1 month under suitable conditions for germ cells maturation. Scallops were placed in experimental raceways supplied with 1 µm-filtered running seawater at 17 ± 1.0°C and fed with a mixed diet of two microalgae (Chaetoceros gracilis and Tisochrysis lutea).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated by following the Extract-all manufacturer instructions and combining the RNeasy Mini Kit (Qiagen) for the nucleic acid purification. A DNAse treatment was also carried out (Qiagen).
|
| Label |
Cyanine 3
|
| Label protocol |
Amplified and labeled according to Agilent Low input One-Color Microarray-Based Gene Expression Analysis protocol
|
| |
|
| Hybridization protocol |
Hybridized according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
| Scan protocol |
Array was scanned according to standard Agilent protocol at a resolution of 2 microns modifying default setting to scan the slide twice at two different sensitivity settings (XDRHi 100% and XDRLo 10%)
|
| Description |
Oocyte RNA of Pecten maximus
|
| Data processing |
Data processed with FeatureExtraction v10.7.3.1 and normalized by quantile normalization using R statistical software
|
| |
|
| Submission date |
Nov 29, 2016 |
| Last update date |
Feb 06, 2017 |
| Contact name |
Massimo Milan |
| E-mail(s) |
[email protected]
|
| Phone |
+39 0498272941
|
| Organization name |
University of Padova
|
| Department |
Dept. of Department of Public Health, Comparative Pathology,and Veterinary Hygiene
|
| Street address |
Viale dell'Università 16
|
| City |
Legnaro |
| State/province |
PD |
| ZIP/Postal code |
35020 |
| Country |
Italy |
| |
|
| Platform ID |
GPL22720 |
| Series (1) |
| GSE90679 |
Transcriptomic features of Pecten maximus oocyte quality and maturation |
|
