|
| Status |
Public on Nov 07, 2007 |
| Title |
Hsp-GAL4>EYA-Wildtype-heatshocked-1 |
| Sample type |
RNA |
| |
|
| Source name |
EYA-Wildtype head, heatshocked 1hr@ 37C, recovered 3hrs @25C
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Hsp-Gal4>UAS-EYA-WT(Transgene IV) adult male and female flies
|
| Treatment protocol |
Adult flies of the above genotypes were subjected to a 1hr heatshock at 37C and then allowed to recover for 3hrs at 25C.
|
| Growth protocol |
Adult male and female flies were raised at 25C. W1118 was used as a control. HspGal flies were crossed to flies carrying one of the folowing transgenes, UAS-EYA-wildtype, UAS-EYA-D493N or UAS-EYA-E728Q and flies carrying both the driver and UAS-EYA trangene were collected
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Flies were transferred to falcon tubes, frozen in liquid nitrogen and vortexed, This was repeated two more times and fly heads were collected using a paint brush from an Rnase-free plate. RNA was isolated from 200 adult heads (4 groups of 50) in Trizol according to the manufacturer's protocol and RNA was cleaned up using the Qiagen RNeasy kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Chips were scanned using the GeneChip Scanner 3000 enabled for High-Resolution scanning.
|
| Description |
wildtype with overexpressed eya transgene
|
| Data processing |
GeneChip Operating Software (GCOS) v1.1.1. Global mean normalization was performed for samples, scaled so that each median is 100. Detection P values were determined from raw data using GCOS software.
|
| |
|
| Submission date |
Nov 06, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Ilaria Rebay |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
University of Chicago
|
| Department |
Ben May Department for Cancer Research
|
| Street address |
929 East 57th Street Room W340
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE9538 |
Identification of Transcriptional Targets Dual Functions Transcription Factor/Phosphatase Eyes Absent |
|
| Relations |
| Reanalyzed by |
GSE119084 |
