|
| Status |
Public on Dec 10, 2016 |
| Title |
SEA treated CD11b+CD103+ lymph DCs, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
FACS purified lymph migrating DC subset incubated with SEA for 18 hours
|
| Organism |
Mus musculus |
| Characteristics |
tissue: intestinal lymph from MLNx animals
cell type: CD11b+CD103+ lymph DCs
treatment: SEA
strain: C57BL/6
|
| Treatment protocol |
30,000 cells of each subset (three replicates) were incubated with or without 15 µg/ml SEA in X-vivo 15 media (Lonza) supplemented with 1% L-glutamine (Invitrogen) and 0.1% 2-mercaptoethanol (Sigma-Aldrich) for 18 hours at 37C and 5% CO2.
|
| Growth protocol |
DC subsets were FACS purified from the lymph of C57BL/6 MLNx mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the RNeasy micro kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
RNA samples were amplified from 20-30 ng total RNA using the Affymetrix WT Pico kit according to the manufacturers’ instructions.
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5ug ss-cDNA were hybridized for 16hr at 45C on Mouse Transcriptome Array 1.0 (Affymetrix) in the Affymetrix GeneChip® Hybridization Oven 645. Microarrays were washed and stained in the Affymetrix GeneChip® Fluidics Station 450.
|
| Scan protocol |
Microarrays were scanned using the Affymetrix GeneChip® Scanner 3000 7G according to the manufacturers’ instructions.
|
| Description |
SEA_DP_3
|
| Data processing |
affymetrix-algorithm-name = rma-gene-full
affymetrix-algorithm-version = 1.0
affymetrix-array-type = MTA-1_0
program-name = Expression Console
program-version = 1.3.1.187
program-company = Affymetrix
|
| |
|
| Submission date |
Dec 09, 2016 |
| Last update date |
Dec 10, 2016 |
| Contact name |
Simon Milling |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0)141 330 6419
|
| Organization name |
University of Glasgow
|
| Department |
Institute of Infection, Immunity and Inflammation
|
| Lab |
Sir Graeme Davies Building
|
| Street address |
120 University Place
|
| City |
Glasgow |
| ZIP/Postal code |
G12 8TA |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE91381 |
Distinct populations of CD11b+ IRF4+ dendritic cells drive Th2 responses in the small intestine and colon |
|
