|
| Status |
Public on Apr 16, 2008 |
| Title |
Resistant, replicate 2 (294_2) |
| Sample type |
RNA |
| |
|
| Source name |
Tumor
|
| Organism |
Homo sapiens |
| Characteristics |
Human pancreatic tumor was xenografted in six-week-old female athymic nude mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
validation
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Nov 13, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Aik Choon Tan |
| E-mail(s) |
[email protected]
|
| Phone |
410-502-8743
|
| Fax |
410-614-9006
|
| Organization name |
Johns Hopkins University
|
| Department |
The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins
|
| Street address |
1650 Orleans Street, RM 1M86
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21231 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE9599 |
Coordinated over-expression of genes in the EGFR pathway predicts sensitivity to EGFR inhibition in pancreatic cancer |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
