|
| Status |
Public on Dec 18, 2008 |
| Title |
HMEC Lactosis 5 |
| Sample type |
RNA |
| |
|
| Source name |
Human Mammary Epithelial Cell
|
| Organism |
Homo sapiens |
| Characteristics |
HMEC in media with 25mM sodium lactate under normoxia
|
| Treatment protocol |
The first set of experiments were done in triplicate by exposing cells to control, 25mM lactic acidosis of pH6.7, 2%hypoxia, and the combination of lactic acidosis and hypoxia. The second set of experiments were done in replica of six replicates and cells were exposed to control, acidosis with the addition of HCl to reach pH 6.7 and 25mM sodium lactate.
|
| Growth protocol |
Human mammary epithelial cells were induced into replicative arrest by growth factor/serum withdrawl for 24 hrs and then exposed to different cultural environments.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAs were extracted with miRVana kit from Ambion
|
| Label |
biotin
|
| Label protocol |
Affymetrix standard protocol
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
Affymetrix standard protocol
|
| Description |
gene expression data from HMEC cells
|
| Data processing |
Data was normalized with RMA and MAS5 and further analyzed with hierarchical clustering as well as binary regression.
|
| |
|
| Submission date |
Nov 20, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Julia, Ling-Yu Chen |
| Organization name |
Duke university
|
| Street address |
CIEMAS Rm.2141, 101 Science Dr.
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE9649 |
Expression studies of HMEC exposed to lactic acidosis and hypoxia |
|
| Relations |
| Reanalyzed by |
GSE119087 |
