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Sample GSM2445401 Query DataSets for GSM2445401
Status Public on Jan 13, 2017
Title RKO.MT1sh49
Sample type RNA
 
Channel 1
Source name RKO.MT1sh49
Organism Homo sapiens
Characteristics cell type: colorectal cancer cells
treatment: DNMT1 shRNA#49 knockdown
cell line: RKO
Growth protocol standard as recommended by ATCC
Extracted molecule total RNA
Extraction protocol Trizol method
Label Cy3
Label protocol standard as recommended by Agilent
 
Channel 2
Source name RKO
Organism Homo sapiens
Characteristics cell type: colorectal cancer cells
cell line: RKO
treatment: non-silencing conrol
Growth protocol standard as recommended by ATCC
Extracted molecule total RNA
Extraction protocol Trizol method
Label Cy5
Label protocol standard as recommended by Agilent
 
 
Hybridization protocol standard as recommended by Agilent
Scan protocol standard as recommended by Agilent
Description DNMT1 shRNA#49 knockdown in RKO cells
Data processing Data were normalized using loess and aquantile methods as described in the Bioconductor limma package
 
Submission date Jan 04, 2017
Last update date Jan 13, 2017
Contact name Stephen B Baylin
E-mail(s) [email protected]
Organization name Johns Hopkins University
Street address 1650 Orleans St. Suite 541
City Baltimore
State/province MD
ZIP/Postal code 21209
Country USA
 
Platform ID GPL6480
Series (2)
GSE93136 Cancer cells with genetic disruption of DNA methyltransferases
GSE93142 Gene expression profiling of cancer cells with genetic disruption of DNA methyltransferases [Agilent-014850]

Data table header descriptions
ID_REF
VALUE Normalized log2 ratio, ch1(test)/ch2(reference)

Data table
ID_REF VALUE
A_23_P100001 -0.099525865
A_23_P100011 -0.715828717
A_23_P100022 -0.18907339
A_23_P100056 -0.467098303
A_23_P100074 -0.998066715
A_23_P100092 0.44492014
A_23_P100103 0.394909356
A_23_P100111 -0.825327115
A_23_P100127 0.238347369
A_23_P100133 -0.371547107
A_23_P100141 0.20356497
A_23_P100156 0.966970902
A_23_P100177 -0.163118531
A_23_P100189 0.161635396
A_23_P100196 0.24615989
A_23_P100203 -0.770836006
A_23_P100220 -0.685005432
A_23_P100240 -0.219757592
A_23_P10025 0.46727254
A_23_P100263 -0.458551781

Total number of rows: 41000

Table truncated, full table size 999 Kbytes.




Supplementary file Size Download File type/resource
GSM2445401_GE_V1_RKO-Cy5_RKODNMT1KD-Cy3.txt.gz 15.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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