|
| Status |
Public on Jan 13, 2017 |
| Title |
HCT116.shUHRF1.d14(KD60) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
HCT116.shUHRF1.d14(KD60)
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: colorectal cancer cells
treatment: UHRF1 shRNA knockdown
cell line: HCT116 (knockdown efficiency 60%)
|
| Growth protocol |
standard as recommended by ATCC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol method
|
| Label |
Cy5
|
| Label protocol |
standard as recommended by Agilent
|
| |
|
| Channel 2 |
| Source name |
HCT116
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: colorectal cancer cells
cell line: HCT116
treatment: non-silencing control
|
| Growth protocol |
standard as recommended by ATCC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol method
|
| Label |
Cy3
|
| Label protocol |
standard as recommended by Agilent
|
| |
|
| |
| Hybridization protocol |
standard as recommended by Agilent
|
| Scan protocol |
standard as recommended by Agilent
|
| Description |
UHRF1 shRNA knockdown in HCT116 cells (knockdown efficiency 60%)
|
| Data processing |
Data were normalized using loess and aquantile methods as described in the Bioconductor limma package
|
| |
|
| Submission date |
Jan 04, 2017 |
| Last update date |
Jan 13, 2017 |
| Contact name |
Stephen B Baylin |
| E-mail(s) |
[email protected]
|
| Organization name |
Johns Hopkins University
|
| Street address |
1650 Orleans St. Suite 541
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21209 |
| Country |
USA |
| |
|
| Platform ID |
GPL6480 |
| Series (2) |
| GSE93136 |
Cancer cells with genetic disruption of DNA methyltransferases |
| GSE93142 |
Gene expression profiling of cancer cells with genetic disruption of DNA methyltransferases [Agilent-014850] |
|
