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Sample GSM24493

Query DataSets for GSM24493

Status

Public on Aug 01, 2005

Title

RHE2_T1_R1, Control Monolayer

Sample type

RNA

Source name

Kidney 293 HEK Cells

Organism

Homo sapiens

Extracted molecule

total RNA

Description

60-70% confluent Human embryonic kidney cell were split into suspension overagarose to form spheroids and grown for an additional 4 days insuspension over agarose prepared with 10% FBS supplemented DMEM
Following growth, media was removed and the spheroids were stored on thesurface of the agarose in vacuum sealed flasks for a 2 week interval at roomtemperature in the dark.
The samples were compared against unstored controls of both spheroids andmonolayer (at 60-70% confluence)samples. All samples were conducted intriplicate.
Rehydration was conducted with DMEM (Invitrogen) containing 10%FBS up tothe final 72hr rehydration timepoint.

Submission date

Jun 03, 2004

Last update date

Aug 22, 2008

Contact name

Richard F. Helm

E-mail(s)

[email protected]

Phone

540-231-4088

Fax

540-231-9070

URL

http://vigen.biochem.vt.edu

Organization name

Virginia Polytechnic Institute and State University

Department

Biochemistry, Fralin Biotechnology Center

Lab

Virginia Tech Center for Genomics

Street address

West Campus Drive

City

Blacksburg

State/province

ZIP/Postal code

24061

Country

USA

Platform ID

GPL96

Series (1)

GSE1455

Two week Storage and Rehydration of HEK 293 Cells at Ambient Temperature

Data table header descriptions
ID_REF
VALUE	'signal' a measure of the abundance of a transcript
BASELINE	baseline intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	BASELINE	ABS_CALL	DETECTION P-VALUE
1007_s_at	3771	3760	P	0.00261695
1053_at	4125	4999	P	0.000321521
117_at	1256	1242	A	0.107301
121_at	6782	6560	P	0.00418033
1255_g_at	343	343	A	0.397994
1294_at	1573	1459	A	0.204022
1316_at	871	852	P	0.00261695
1320_at	691	659	A	0.284747
1405_i_at	317	310	A	0.900939
1431_at	403	370	P	0.0193035
1438_at	2153	2074	A	0.267463
1487_at	3005	3048	P	0.0130918
1494_f_at	1551	1529	A	0.0738301
1598_g_at	5662	5428	P	0.0313357
160020_at	3368	3147	P	0.0439678
1729_at	1705	1633	P	0.0193035
1773_at	1970	1953	P	0.0149366
177_at	817	891	M	0.0604193
179_at	7776	7984	P	0.00868918
1861_at	1179	1155	P	0.0170005

Total number of rows: 22283

Table truncated, full table size 703 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM24493.CEL.gz	4.1 Mb	(ftp)(http)	CEL
GSM24493.DAT.gz	36.6 Mb	(ftp)(http)	DAT
GSM24493.EXP.gz	313 b	(ftp)(http)	EXP