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Sample GSM24496

Query DataSets for GSM24496

Status

Public on Aug 01, 2005

Title

RHE2_T2_R1, Spheroid prior to storage

Sample type

RNA

Source name

Kidney 293 HEK Cells

Organism

Homo sapiens

Extracted molecule

total RNA

Description

60-70% confluent Human embryonic kidney cell were split into suspension overagarose to form spheroids and grown for an additional 4 days insuspension over agarose prepared with 10% FBS supplemented DMEM
Following growth, media was removed and the spheroids were stored on thesurface of the agarose in vacuum sealed flasks for a 2 week interval at roomtemperature in the dark.
The samples were compared against unstored controls of both spheroids andmonolayer (at 60-70% confluence)samples. All samples were conducted intriplicate.
Rehydration was conducted with DMEM (Invitrogen) containing 10%FBS up tothe final 72hr rehydration timepoint.

Submission date

Jun 03, 2004

Last update date

Aug 22, 2008

Contact name

Richard F. Helm

E-mail(s)

[email protected]

Phone

540-231-4088

Fax

540-231-9070

URL

http://vigen.biochem.vt.edu

Organization name

Virginia Polytechnic Institute and State University

Department

Biochemistry, Fralin Biotechnology Center

Lab

Virginia Tech Center for Genomics

Street address

West Campus Drive

City

Blacksburg

State/province

ZIP/Postal code

24061

Country

USA

Platform ID

GPL96

Series (1)

GSE1455

Two week Storage and Rehydration of HEK 293 Cells at Ambient Temperature

Data table header descriptions
ID_REF
VALUE	'signal' a measure of the abundance of a transcript
BASELINE	baseline intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	BASELINE	ABS_CALL	DETECTION P-VALUE
1007_s_at	5043	3760	P	0.0011411
1053_at	6025	4999	P	0.000218889
117_at	1116	1242	A	0.150527
121_at	5751	6560	P	0.0114474
1255_g_at	319	343	A	0.0668649
1294_at	1341	1459	A	0.162935
1316_at	719	852	P	0.0218664
1320_at	661	659	A	0.250724
1405_i_at	287	310	A	0.864026
1431_at	357	370	P	0.0149366
1438_at	1833	2074	A	0.418069
1487_at	3052	3048	P	0.0114474
1494_f_at	1396	1529	P	0.0278597
1598_g_at	5063	5428	P	0.0278597
160020_at	2485	3147	A	0.105738
1729_at	1596	1633	P	0.000804598
1773_at	1863	1953	P	0.0114474
177_at	1061	891	P	0.00754291
179_at	7667	7984	P	0.00993898
1861_at	1196	1155	P	0.00998544

Total number of rows: 22283

Table truncated, full table size 705 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM24496.CEL.gz	4.1 Mb	(ftp)(http)	CEL
GSM24496.DAT.gz	37.0 Mb	(ftp)(http)	DAT
GSM24496.EXP.gz	314 b	(ftp)(http)	EXP