GEO Accession viewer

NCBI > GEO > Accession Display

Not logged in | Login

GEO help: Mouse over screen elements for information.

Sample GSM24498

Query DataSets for GSM24498

Status

Public on Aug 01, 2005

Title

RHE2_T2_R3, Spheroid prior to storage

Sample type

RNA

Source name

Kidney 293 HEK Cells

Organism

Homo sapiens

Extracted molecule

total RNA

Description

60-70% confluent Human embryonic kidney cell were split into suspension overagarose to form spheroids and grown for an additional 4 days insuspension over agarose prepared with 10% FBS supplemented DMEM
Following growth, media was removed and the spheroids were stored on thesurface of the agarose in vacuum sealed flasks for a 2 week interval at roomtemperature in the dark.
The samples were compared against unstored controls of both spheroids andmonolayer (at 60-70% confluence)samples. All samples were conducted intriplicate.
Rehydration was conducted with DMEM (Invitrogen) containing 10%FBS up tothe final 72hr rehydration timepoint.

Submission date

Jun 03, 2004

Last update date

Aug 22, 2008

Contact name

Richard F. Helm

E-mail(s)

[email protected]

Phone

540-231-4088

Fax

540-231-9070

URL

http://vigen.biochem.vt.edu

Organization name

Virginia Polytechnic Institute and State University

Department

Biochemistry, Fralin Biotechnology Center

Lab

Virginia Tech Center for Genomics

Street address

West Campus Drive

City

Blacksburg

State/province

ZIP/Postal code

24061

Country

USA

Platform ID

GPL96

Series (1)

GSE1455

Two week Storage and Rehydration of HEK 293 Cells at Ambient Temperature

Data table header descriptions
ID_REF
VALUE	'signal' a measure of the abundance of a transcript
BASELINE	baseline intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	BASELINE	ABS_CALL	DETECTION P-VALUE
1007_s_at	4255	3760	P	0.000804598
1053_at	6265	4999	P	0.000218889
117_at	1111	1242	A	0.204022
121_at	5742	6560	P	0.00486276
1255_g_at	342	343	A	0.11716
1294_at	1435	1459	A	0.089405
1316_at	828	852	P	0.0170005
1320_at	598	659	A	0.438361
1405_i_at	305	310	A	0.911443
1431_at	381	370	P	0.00418033
1438_at	1955	2074	A	0.397994
1487_at	2462	3048	P	0.0130918
1494_f_at	1502	1529	P	0.0393654
1598_g_at	5153	5428	P	0.0393654
160020_at	2647	3147	A	0.204022
1729_at	1659	1633	P	0.00868918
1773_at	1867	1953	P	0.0313357
177_at	1061	891	P	0.0149366
179_at	7160	7984	P	0.0313357
1861_at	1169	1155	P	0.0130918

Total number of rows: 22283

Table truncated, full table size 704 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM24498.CEL.gz	4.0 Mb	(ftp)(http)	CEL
GSM24498.DAT.gz	36.0 Mb	(ftp)(http)	DAT
GSM24498.EXP.gz	314 b	(ftp)(http)	EXP