|
| Status |
Public on Aug 15, 2017 |
| Title |
2 Metastatic Colorectal Cancer |
| Sample type |
RNA |
| |
|
| Source name |
Paraffin-embedded metastatic colorectal cancer tissues
|
| Organism |
Homo sapiens |
| Characteristics |
developmental stage: metastatic
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were isolated from paraffin-embedded human colorectal cancer tissues using mirVanaTM RNA Isolation Kit (AM1561) according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Total RNA were dephosphorylated, denaturated, and then labeled with Cyanine3-pCp.
|
| |
|
| Hybridization protocol |
The labeled RNA were hybridized for 20 hr at 55°C on Agilent-038166 Array. After hybridization, microarrays were washed 5 minute at room temperature with GE Wash Buffer 1 (Agilent) and 5 minute with 37°C GE Wash buffer 2 (Agilent).
|
| Scan protocol |
After washing, the arrays were scanned with the Agilent Scanner G2505C (Agilent Technologies).
|
| Description |
Paraffin-embedded human metastatic colorectal cancer tissues
|
| Data processing |
Feature Extraction software (version10.7, Agilent Technologies) was used to analyze array images to get raw data. Next, Genespring software (version 10.0; Agilent Technologies) was employed to finish the basic analysis with the raw data according to scale to mean of all samples.
Differentially expressed miRNAs were identified through fold change as well as P value calculated using t-test.
|
| |
|
| Submission date |
Jan 10, 2017 |
| Last update date |
Aug 15, 2017 |
| Contact name |
Yihua Bei |
| E-mail(s) |
[email protected]
|
| Phone |
+86 13816265303
|
| Organization name |
Shanghai University
|
| Department |
School of Life Science
|
| Lab |
Regeneration Lab
|
| Street address |
133 Nanchen Road
|
| City |
Shanghai |
| ZIP/Postal code |
200444 |
| Country |
China |
| |
|
| Platform ID |
GPL16543 |
| Series (1) |
| GSE93377 |
microRNA data in human metastatic versus non-metastatic colorectal cancer tissues |
|
