|
| Status |
Public on Mar 09, 2017 |
| Title |
Fkh1-Myc_fkh2Δ_G1_anti_Myc_rep_1 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
ZOy48
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype/variation: FKH1-MYC9 (TRP1) bar1Δ::hisG fkh2Δ::HIS3MX
chip antibody: 9E10 monoclonal
|
| Treatment protocol |
incubated with α-factor for 3hrs
|
| Growth protocol |
cells were grown in YEPD
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted as described in as described in Viggiani CJ, Aparicio JG, Aparicio OM (2009) ChIP-chip to analyze the binding of replication proteins to chromatin using oligonucleotide DNA microarrays. Methods Mol Biol 521: 255-278.
|
| Label |
Cy5
|
| Label protocol |
Nimblegen standard protocol
|
| |
|
| Channel 2 |
| Source name |
ZOy48
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype/variation: FKH1-MYC9 (TRP1) bar1Δ::hisG fkh2Δ::HIS3MX
chip antibody: none
|
| Treatment protocol |
incubated with α-factor for 3hrs
|
| Growth protocol |
cells were grown in YEPD
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted as described in as described in Viggiani CJ, Aparicio JG, Aparicio OM (2009) ChIP-chip to analyze the binding of replication proteins to chromatin using oligonucleotide DNA microarrays. Methods Mol Biol 521: 255-278.
|
| Label |
Cy3
|
| Label protocol |
Nimblegen standard protocol
|
| |
|
| |
| Hybridization protocol |
Nimblegen standard protocol
|
| Scan protocol |
Genepix Personal 4100A Scanner (Axon Instruments).
|
| Description |
ChIP-chip FKH1-MYC9 G1-Phase cells
|
| Data processing |
We used MA2C to perform normalization.
|
| |
|
| Submission date |
Jan 11, 2017 |
| Last update date |
Mar 09, 2017 |
| Contact name |
Andrew Zachary Ostrow |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Southern California
|
| Department |
Biological Sciences
|
| Lab |
Oscar Aparicio
|
| Street address |
1050 Childs Way
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90089 |
| Country |
USA |
| |
|
| Platform ID |
GPL14887 |
| Series (2) |
| GSE93518 |
Conserved forkhead dimerization motif controls DNA replication timing and spatial organization of chromosomes in S. cerevisiae (ChIP-chip) |
| GSE94796 |
Conserved forkhead dimerization motif controls DNA replication timing and spatial organization of chromosomes in S. cerevisiae |
|
