|
| Status |
Public on Jul 25, 2017 |
| Title |
Mutant Sample, Biological Replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
P17 striatal tissue, Drd2Cre;Mapk3(Erk1)-/-; Mapk1(Erk2)Floxed/Floxed mouse
|
| Organism |
Mus musculus |
| Characteristics |
age: Postnatal day 17
Sex: male
genotype: Drd2Cre;Mapk3(Erk1)-/-; Mapk1(Erk2)Floxed/Floxed
tissue: striatum
|
| Treatment protocol |
Mice were cervically dislocated and the brain removed and placed in ice-cold 1x PBS. 1mm coronal sections were made using a chilled acrylix brain matrix. 1mm diameter punches were then taken from dorsal striatum and flash frozen using liquid nitrogen
|
| Growth protocol |
Striatal punches were extracted from control and mutant mice at postnatal day 17
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol reagent (Invitrogen) followed by mRNA extraction using an RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Transcriptome Array 1.0. GeneChips were washed and stained in the Genechip Fluidics Station 450.
|
| Scan protocol |
Gene Chips were scanned using Affymetrix GeneChip Scanner 3000 7G Plus Targeted Genotyping System with Autoloader
|
| Description |
Gene Expression Data from P17 striatum of ERK knockout mice
|
| Data processing |
Data was analyzed using Affymetrix Transcriptome Analysis Console 3 using default settings. RMA normalization.
|
| |
|
| Submission date |
Jan 19, 2017 |
| Last update date |
Jul 25, 2017 |
| Contact name |
Scott Hutton |
| Organization name |
University of North Carolina
|
| Department |
Neuroscience Center
|
| Lab |
William Snider Lab
|
| Street address |
115 Mason Farm Rd. Rm 8131
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE93844 |
Changes in gene expression in the striatum of Drd2Cre; ERK1/ERK2 knockout mice |
|
