|
| Status |
Public on Jul 23, 2017 |
| Title |
10023-SZ-L5-R2 |
| Sample type |
RNA |
| |
|
| Source name |
dorsolateral prefrontal cortex
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: dorsolateral prefrontal cortex
cell type: pyramidal neuron cell
dsm-iv diagnoses: Disorganized schizophrenia
|
| Treatment protocol |
An independent committee of experienced research clinicians made consensus DSM-IV diagnoses for all 72 subjects on the basis of medical records and structured diagnostic interviews conducted with the decedent’s family members.
|
| Growth protocol |
Tissue sections (12 μm) containing DLPFC area 9 were cut on a cryostat, mounted on glass polyethylene naphthalate membrane slides (Leica Microsystems, Bannockburn, IL) which were blinded as to diagnosis, and stained with thionin for Nissl substance.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For each sample, RNA was extracted using the QIAGEN® RNeasy Plus Micro kit (QIAGEN, Valencia).
|
| Label |
Encore Biotin module (Nugen)
|
| Label protocol |
Labeled using the Encore Biotin module (Nugen).
|
| |
|
| Hybridization protocol |
cDNA was synthesized and amplified using the Ovation Pico WTA System
|
| Scan protocol |
loaded on an Affymetrix GeneChip® HT HG-U133+ PM Array Plate
|
| Description |
pair 11, layer L5, diagnosis Schiz, replicate R2
Gene expression data for pair 11, layer L5, diagnosis Schiz, replicate R2
|
| Data processing |
Expression intensities were extracted from Affymetrix Expression Console and normalized using Robust Multi-array Average Express.
|
| |
|
| Submission date |
Jan 24, 2017 |
| Last update date |
Jul 23, 2017 |
| Contact name |
David Lewis |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Pittsburgh
|
| Department |
Psychiatry and Neuroscience
|
| Street address |
3811 O'Hara St
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
|
| Platform ID |
GPL13158 |
| Series (1) |
| GSE93987 |
Distinctive transcriptome alterations of prefrontal pyramidal neurons in schizophrenia and schizoaffective disorder |
|
