|
| Status |
Public on Dec 06, 2008 |
| Title |
BEAS-2B treated with MTX Array 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
BEAS-2B treated MTX labeled with Cy5
|
| Organism |
Homo sapiens |
| Characteristics |
Cell line : BEAS-2B
(human normal bronchial cells)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction
|
| Label |
Cy5
|
| Label protocol |
Stratagene FairPlay III microarray labeling kit
|
| |
|
| Channel 2 |
| Source name |
BEAS-2B treated DMSO labeled with Cy3
|
| Organism |
Homo sapiens |
| Characteristics |
Cell line : BEAS-2B
(human normal bronchial cells)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction
|
| Label |
Cy3
|
| Label protocol |
Stratagene FairPlay III microarray labeling kit
|
| |
|
| |
| Hybridization protocol |
Stratagene FairPlay III microarray labeling kit
|
| Scan protocol |
PixelSize=5
DateTime=2006/10/02 10:03:38
Scanner=GenePix 4000B [81993]
Scanning software = GenePix 4.1 software
|
| Description |
MTX vs Control
|
| Data processing |
The fluorescence intensity of each spot was calculated by local median background subtraction. We used the robust scatter-plot smoother LOWESS function to perform intensity-dependent normalization of gene expression. Scatter-plot analysis was performed using Microsoft Excel 2000 ). A significance analysis of microarray (SAM) was performed for genes with significant changes in expression
|
| |
|
| Submission date |
Dec 06, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
mee song |
| E-mail(s) |
[email protected]
|
| Organization name |
KIST
|
| Street address |
P.O. Box 131, cheongryang
|
| City |
seoul |
| ZIP/Postal code |
136-791 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL1708 |
| Series (1) |
| GSE9815 |
Microarray Analysis of Differentially Expressed Genes in human bronchial cells(BEAS-2B) treated MTX |
|
