100ng of total RNA were amplified and labeled using the Whole Transcript (WT) Sense Target Labeling Protocol
Hybridization protocol
Affymetrix GeneChip® Human Gene 1.0 ST arrays were hybridized to 11 mg of labeled, amplified cDNA, washed, stained and scanned according to the protocol described in WT Sense Target Labeling Assay Manual (Version 4; FS450_0007)
Scan protocol
Scanning was done on an Affymetrix GeneChip Scanner 7G
Description
Samples labeling and hybridization day 2, replicate 2
Data processing
Normalized expression signals were calculated from Affymetrix CEL files using RMA (Irizarry et al., 2003).
