|
| Status |
Public on Dec 12, 2007 |
| Title |
TRT_compound A_4h_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
A2780 human ovarian cancer cell line treated with compound A
|
| Organism |
Homo sapiens |
| Characteristics |
compound: A
time: 4h
day: A
|
| Treatment protocol |
1X10E6 cells were plated in 100mm plates and after 24h were exposed to the different compounds
|
| Growth protocol |
A2780 cells were grown in RPMI 1640 medium (Gibco Invitrogen) + 10% Fetal Bovine Serum (Euroclone) and split before confluence seeding approx. 1X10E5 cells/ml.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNeasy Mini kit (Qiagen, catalog no. 74106) according to manufacturer recommendation
|
| Label |
biotin
|
| Label protocol |
RNA samples were labelled using GeneChip Expression 3’Amplification One cycle target labeling and control reagents kit (Affymetrix, part no = 900493), following standard protocol
|
| |
|
| Hybridization protocol |
cRNA fragmented samples were hybridized on Affymetrix HGU133plus2 chips for 16h in Genechip Hybridization Oven 640 (Affymetrix), wash and staining were performed on Genechip Fluidics Station 450 (Affymetrix) following standard procedure
|
| Scan protocol |
Hybridized chips were scanned using Affymetrix Genechip Scanner 3000
|
| Description |
A2780 human ovarian cancer cell line treated with compound A, collected after 4h
replicate 2
|
| Data processing |
Data were analyzed with RMA, day to day variation has been considered in the analysis using a liner model based approach
|
| |
|
| Submission date |
Dec 10, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Roberta Bosotti |
| E-mail(s) |
[email protected]
|
| Organization name |
Nerviano Medical Sciences srl
|
| Department |
Biotechnology
|
| Lab |
Genomics
|
| Street address |
viale Pasteur,10
|
| City |
Nerviano |
| State/province |
MI |
| ZIP/Postal code |
20014 |
| Country |
Italy |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE9826 |
Summarizing probe levels of Affymetrix arrays taking into account day-to-day variability |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
