|
| Status |
Public on Feb 12, 2008 |
| Title |
OTSCC normal control 1 |
| Sample type |
RNA |
| |
|
| Source name |
Tongue Squamous Cells from control subjects
|
| Organism |
Homo sapiens |
| Characteristics |
Sex M, Age 41
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was isolated using RNeasy Mini kit (Qiagen), and quantified by the RiboGreen RNA Quantitation Reagent (Molecular Probes).
|
| Label |
biotin
|
| Label protocol |
The Enzo BioArray High Yield RNA Transcript Labeling System (Enzo) was used for labeling the sample prior to hybridization. The biotinylated cRNA (IVT product) was purified using the RNeasy kit (Qiagen).
|
| |
|
| Hybridization protocol |
The samples were hybridized to the Affymetrix Human Genome U133 Plus 2.0 GeneChip arrays according to the Affymetrix protocols.
|
| Scan protocol |
The arrays were scanned with a GeneChip Scanner 3000. The scanned array images were processed with GeneChip Operating software (GCOS), and the CEL files were extracted for further analysis.
|
| Description |
This studys aim is to compare specific transcriptomic signatures that are associated with Oral Tongue Squamous Cell Carcinoma
|
| Data processing |
RMA
|
| |
|
| Submission date |
Dec 11, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Tianwei Yu |
| Organization name |
Emory University
|
| Department |
Biostatistics
|
| Street address |
1518 Clifton Rd NE
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE9844 |
Transcriptomic Dissection of Tongue Squamous Cell Carcinoma |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
