|
Status |
Public on Mar 01, 2008 |
Title |
X2227 |
Sample type |
RNA |
|
|
Source name |
Carcinoma of ovary, fallopian tube or peritonem
|
Organism |
Homo sapiens |
Characteristics |
Primary.Site : Ovary Type : Malignant Subtype : Endo StageCode : NA Consolidated.Grade : NA
|
Treatment protocol |
Frozen tissue stored at -80oC taken after surgery
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction and column chromotogrpahy
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using Affymetrix GS300-7G scanner
|
Description |
Gene expression data from human ovarian carcinoma
|
Data processing |
The data were analyzed with Gene Chip Operating Software Version 1.4 using Affymetrix default analysis settings. The CEL files generated was later normalized using the RMA method
|
|
|
Submission date |
Dec 14, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Prof David Bowtell PhD. |
E-mail(s) |
[email protected]
|
Phone |
+613 96561287
|
Fax |
+613 96561414
|
URL |
http://www.petermac-research.org.au
|
Organization name |
PeterMacCallum Cancer Centre
|
Department |
Research Division
|
Street address |
Locked Bag 1 A' Beckett St
|
City |
Melbourne |
State/province |
VIC |
ZIP/Postal code |
8006 |
Country |
Australia |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE9891 |
Expression profile of 285 ovarian tumour samples |
GSE9899 |
Expression profile of ovarian tumour samples |
|
Relations |
Reanalyzed by |
GSE40785 |
Reanalyzed by |
GSE64985 |
Reanalyzed by |
GSE119087 |