|
| Status |
Public on Mar 27, 2018 |
| Title |
aERKO-veh 3 |
| Sample type |
RNA |
| |
|
| Source name |
aERKO mouse SVs control group
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6
genotype/variation: estrogen receptor-alpha knockout
gender: male
tissue: seminal vesicles
treatment: vehicle
|
| Treatment protocol |
For neonatal treatment, male pups were treated by subcutaneous injection with DES (Sigma Aldrich), dissolved in corn oil at 2 µg/pup/day on days 1-5 (day of birth = 1) (DES group) or an equal volume of corn oil (control group).
|
| Growth protocol |
The SV tissues were collected at week 10 from WT and aERKO mice in the control and DES groups.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Three hundred nanograms (300 ng) of total RNA were amplified and labeled as directed using the Affymetrix WT Plus Reagent Kit (WT Plus Kit).
|
| |
|
| Hybridization protocol |
Five and one half micrograms (5.5 μg) of amplified biotin-cDNAs were fragmented and hybridized to each array for 16 hours at 45°C in a rotating hybridization oven. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0001.
|
| Scan protocol |
Arrays were scanned in an Affymetrix Scanner 3000.
|
| Description |
Gene expression data from αERKO mouse SV control group.
|
| Data processing |
Data was obtained using the GeneChip® Expression Console Software (AGCC; Version 1.2) using the RMA algorithm to generate .CHP files.
|
| |
|
| Submission date |
Feb 23, 2017 |
| Last update date |
Mar 29, 2018 |
| Contact name |
Yin Li |
| E-mail(s) |
[email protected]
|
| Organization name |
NIEHS
|
| Street address |
111 TW alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL20775 |
| Series (2) |
| GSE95314 |
Gene expression profiling in the seminal vesicles (SVs) from neonatal DES-treated mice [array] |
| GSE102450 |
Gene expression and DNA methylation profiling in the seminal vesicles (SVs) from neonatal DES-treated mice |
|
