|
| Status |
Public on Jun 16, 2017 |
| Title |
Naïve T-injected severely inflamed colon tissue, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
colon tissue from Naïve T-injected mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: Rag1-/-
treatment: Naïve T-injected
tissue: severely inflamed colons
age: 4-5 mos
|
| Treatment protocol |
Colons were excised and distal colon tissue (from rectum to proximal/distal junction) was snap frozen.
|
| Growth protocol |
Animals were injected with Tregs or naïve T cells at 10 weeks of age and sacrificed 2-4 months after injection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Qiagen RNAeasy tissue microarray tissue mini kit following manufacturers recommended protocol.
|
| Label |
biotin
|
| Label protocol |
The targets were prepared using the Affyemtrix GeneChip WT PlUS Reagent Kit and labeled by biotinylation.
|
| |
|
| Hybridization protocol |
Following labeling, biotinylated cRNA was hybridized to the GeneChip® Mouse Exon 1.0 ST Array for 16 hr at 45C in an Affymetrix HybridizationOven. Hybridized array was washed and stained using fluorescent stain SAPE and Anti-streptavidin antibody.
|
| Scan protocol |
The arrays were scanned on GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from severely inflamed colons
|
| Data processing |
The data were extracted and quantified at the gene level using the Affymetrix expression console with RMA processing.
|
| |
|
| Submission date |
Mar 06, 2017 |
| Last update date |
Jun 17, 2017 |
| Contact name |
Jesse Lyons |
| Organization name |
Massachusetts Institute of Technology
|
| Department |
Bioengineering
|
| Lab |
Douglas Lauffenburger
|
| Street address |
77 Massachusetts Ave 16-343
|
| City |
CAMBRIDGE |
| State/province |
Ma |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE95705 |
Integrated in vivo multiomics analysis identifies p21-activated kinase signaling as a driver of colitis |
|
