|
| Status |
Public on Feb 13, 2018 |
| Title |
KL WT repl 4 |
| Sample type |
RNA |
| |
|
| Source name |
KL WT
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: WT
tissue: Bone Marrow
cell type: KL cells
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Eukaryote Total RNA Pico
|
| Label |
Biotin
|
| Label protocol |
Labellings were performed at Sequencing and Genome Technologies Shared Resource (Duke University) according to standard operative procedures.
|
| |
|
| Hybridization protocol |
Hybridizations were performed at Sequencing and Genome Technologies Shared Resource (Duke University) according to standard operative procedures.
|
| Scan protocol |
Scans were performed at Sequencing and Genome Technologies Shared Resource (Duke University) according to standard operative procedures.
|
| Description |
Gene expression from KL isolated from WT mice
BM-KL4
|
| Data processing |
Partek Genomics Suite 6.6 (Partek Inc., St. Louis, MO) was used to perform data analysis. Robust multi-chip analysis (RMA) normalization was done on the entire data set. Multi-way ANOVA and fold change were performed to select target genes that were differentially expressed between ___ and ___. Top differentially expressed genes were selected with p value cutoff of ___ based on ANOVA test and fold change cutoff of __. Hierarchical Clustering was performed on differentially expressed genes based on Average Linkage with Pearson's Dissimilarity.
|
| |
|
| Submission date |
Mar 06, 2017 |
| Last update date |
Feb 13, 2018 |
| Contact name |
LUIGI RACIOPPI |
| E-mail(s) |
[email protected]
|
| Phone |
(919)6816212
|
| Organization name |
Duke University
|
| Department |
Medicine
|
| Lab |
Cell therapy Hematological Malignancies
|
| Street address |
La Salle, GSRB1 2006
|
| City |
Durham |
| State/province |
North Carolina |
| ZIP/Postal code |
27707 |
| Country |
USA |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE95733 |
Gene expression in WT and Camkk2 KO murine hematopoietic stem cells |
|
