|
| Status |
Public on Mar 14, 2017 |
| Title |
WT23M |
| Sample type |
RNA |
| |
|
| Source name |
Mice liver tissue at 12 weeks of age
|
| Organism |
Mus musculus |
| Characteristics |
strain: Mouse C57BL6/J
tissue: Liver
genotype: ENT3 +/+
|
| Treatment protocol |
Mice were euthanized at 12 weeks of age and liver tissue was snap frozen in liquid nitrogen for RNA extraction
|
| Growth protocol |
Mice liver tissue collected at 12 weeks of age
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Herring sperm DNA, Acetylated BSA (50mg/mL), Eukaryotic Hybridization Controls (Affymetrix) •Heat 650C for 5 minutes, 990C for 5 minutes, 450C for 5 minutes centrifuge top speed for 5 minutes. •Hybridize 16 hours at 450 at 60rpm•Fluidics washing is FS450_0001
|
| Scan protocol |
Affymetrix Gene Chip Scanner 3000 7G using AGCC (Affymetrix GeneChip Command Console)
|
| Description |
Gene expression data from liver of Wildtype and Knockout mice
|
| Data processing |
The data were analyzed with Transcriptome Analysis Console (TAC) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Mar 13, 2017 |
| Last update date |
Mar 14, 2017 |
| Contact name |
Bhawana Dikshit |
| E-mail(s) |
[email protected]
|
| Phone |
6149663558
|
| Organization name |
Ohio State University
|
| Street address |
500 W 12th Avenue
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43202 |
| Country |
USA |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
|
