|
| Status |
Public on Jun 26, 2008 |
| Title |
Standard medium supplemented with 9% ethanol (380 OH 2) |
| Sample type |
RNA |
| |
|
| Source name |
whole body
|
| Organism |
Drosophila melanogaster |
| Characteristics |
380 OH 2
|
| Biomaterial provider |
laboratory of Robert Anholt
|
| Treatment protocol |
Larvae were reared in Ethanol medium prior to RNA extraction
|
| Growth protocol |
under standard conditions 25C, 12:12 light/dark cycle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
Biotinylated cRNA
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 mkg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Larvae were reared on three different media: standard cornmeal-molasses medium, standard medium supplemented with 9% ethanol and standard medium enriched with tomato paste. After eclosion, we transferred all flies to standard medium at 25°C under a 12:12 hour light/dark cycle prior to characterization of behavioral responses to benzaldehyde and transcription profiling.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Jan 02, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Deepa Sambandan |
| E-mail(s) |
[email protected]
|
| Organization name |
North Carolina State University
|
| Department |
Genetics
|
| Street address |
100, Derieux
|
| City |
Raleigh |
| State/province |
NC |
| ZIP/Postal code |
27560 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE10053 |
Gene expression index for olfactory behavior in Drosophila melanogaster |
|
| Relations |
| Reanalyzed by |
GSE119084 |
