|
| Status |
Public on Jan 03, 2009 |
| Title |
Liver, wt ND |
| Sample type |
RNA |
| |
|
| Source name |
Liver, wt, on a normal diet for 6 months
|
| Organism |
Mus musculus |
| Characteristics |
Gender: female
Tissue: liver
Nutritional state: fed mice
|
| Treatment protocol |
Livers were snap-frozen and stored at -80C. Mice were either fed a normal diet (ND) (11% energy from fat) or a high-fat diet (FD) (58% energy from fat) (Research Diets, New Brunswick, NJ; products no D12310 and D12309, respectively).
|
| Growth protocol |
Female mice were sacrificed at 8 months old.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted according to Chomczynski and Sacchi (Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. 1987. Anal Biochem 162: 156-159) and was then purified using an RNeasy Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol, using the Enzo RNA transcript-labeling kit, from 4.8 ug of total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Murine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner GA2500.
|
| Description |
Gene expression data from wildtype mice on ND
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jan 04, 2008 |
| Last update date |
Jan 04, 2008 |
| Contact name |
Céline Fernandez |
| E-mail(s) |
[email protected]
|
| Phone |
+46 46 222 9772
|
| Fax |
+46 46 222 4022
|
| Organization name |
Medical Faculty, Lund University
|
| Department |
Experimental Medical Science
|
| Lab |
Molecular Endocrinology Group
|
| Street address |
BMC C11
|
| City |
Lund |
| ZIP/Postal code |
221 84 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE10067 |
Gene expression data from murine liver samples comparing hormone sensitive lipase (HSL) knockout mice vs. wildtype mice |
|
