|
| Status |
Public on Mar 27, 2018 |
| Title |
Combination sample 1 |
| Sample type |
RNA |
| |
|
| Source name |
pmel1 CD8+ T cells from anti-PD-1/L1 and anti-CD27 treated mice
|
| Organism |
Mus musculus |
| Characteristics |
cell type: CD8+ T cell
treatment: anti-PD-1/L1 and anti-CD27
|
| Treatment protocol |
Adoptively transferred CD8+ T cells were treated in vivo with peptide and anti-CD27, anti-PD-1/L1 or all three together (with isotype controls to equalize antibody dose across groups).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from isolated CD8+Thy1.1+ T cells using Qiagen RNeasy kits and gDNA eliminator columns
|
| Label |
biotin
|
| Label protocol |
cDNA was prepared by AROS Biotechnology (Denmark) using 50ng starting RNA material and labelled
|
| |
|
| Hybridization protocol |
Following fragmentation, cDNA samples were hybridized for 16 hours at 45 degrees on an Affy HTA array and washed and stained using the Affymetrix Hyb. Wash and stain kit all by AROS Biotechnology Ltd
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
A3214_07
Activated CD8+ T cells
|
| Data processing |
RMA normalization, oligo R package (version 1.38.0).
|
| |
|
| Submission date |
Mar 22, 2017 |
| Last update date |
Mar 27, 2018 |
| Contact name |
Sarah Louise Buchan |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Southampton
|
| Department |
Cancer Sciences Unit
|
| Lab |
Tenovus Labs
|
| Street address |
Tremona Road
|
| City |
Southampton |
| State/province |
Hants |
| ZIP/Postal code |
SO16 6YD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE96923 |
Expression data from pmel1 CD8+ T cells stimulated with agonist anti-CD27, blockade of PD-1/L1 or both together |
|
