|
| Status |
Public on Mar 24, 2017 |
| Title |
B. subtilis 3610 midlog Exp.1 rep.1 |
| Sample type |
RNA |
| |
|
| Source name |
Midlog cells
|
| Organism |
Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10 |
| Characteristics |
genotype: Wild Type
|
| Treatment protocol |
No treatment, comparison between parental strain B. subtilis NCIB3610 and an S-adenosylmethionine decarboxylase (speD) mutant
|
| Growth protocol |
Bacterial cultures were grown to midlog phase in MSgg polyamine-free chemically defined medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with an Ambion Ribopure kit.
|
| Label |
biotin
|
| Label protocol |
5 microgram of total RNA was labelled according to Affymetrix standard protocol
|
| |
|
| Hybridization protocol |
Labeled cDNAs were hybridized to Affymetrix antisense Bacillus subtilis microarrays using the suggested Affymetrix protocol
|
| Scan protocol |
Affymetrix standard procedure
|
| Description |
Expression data from midlog planktonic NCIB3610 parental strain
|
| Data processing |
Data was processed with MAS5 using Expression Console software.
|
| |
|
| Submission date |
Mar 23, 2017 |
| Last update date |
Mar 24, 2017 |
| Contact name |
Anthony John Michael |
| E-mail(s) |
[email protected]
|
| Phone |
2146484170
|
| Organization name |
UT Southwestern Medical Center, Dallas
|
| Department |
Pharmacology
|
| Street address |
6001 Forest Park
|
| City |
Dallas |
| State/province |
Texas |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL343 |
| Series (1) |
| GSE96942 |
S-adenosylmethionine decarboxylase-dependent gene expression in planktonic Bacillus subtilis NCIB3610 |
|
