|
Status |
Public on Oct 10, 2009 |
Title |
PBMC_CD3CD28Activated_IL23100ng/mlTreated_Rep2 |
Sample type |
RNA |
|
|
Source name |
PBMC CD3 CD28 Simulated IL23 100ng/ml
|
Organism |
Homo sapiens |
Characteristics |
Cryopreserved PBMC
|
Extracted molecule |
total RNA |
Extraction protocol |
Standard TRIZOL Extraction followed by Qiagen RNeasy Column Purification
|
Label |
Cy3
|
Label protocol |
250ng Total RNA amplified and labelled according to Ambion Illumina TotalPrep RNA Amplification Kit protocol
|
|
|
Hybridization protocol |
1.5ug labelled cRNA Hybridized according to Illumina Standard protocol
|
Scan protocol |
Scanned on Illumina BeadStation
|
Description |
Replicate 2 of 6
|
Data processing |
Data quantile normalised on Illumina BeadStudio Gene Expression Module v3.2.3
|
|
|
Submission date |
Jan 07, 2008 |
Last update date |
Oct 22, 2008 |
Contact name |
Graham Heap |
E-mail(s) |
[email protected]
|
Organization name |
QMUL
|
Street address |
4 Newark Street
|
City |
London |
ZIP/Postal code |
E1 2AT |
Country |
United Kingdom |
|
|
Platform ID |
GPL6102 |
Series (1) |
GSE10080 |
IL-23 100ng/ml Treatment of CD3 CD28 Stimulated PBMCs |
|