Placed in larval packet test papers treated with 0.4% coumaphos. This is approximately a LD25 dosage for each strain. For more details see: Insect Molecular Biology (2007) 16(1), 49-60.
Biomaterial provider
Cattle Fever Tick Research Laboratory (CFTRL)
Treatment protocol
Placed in larval packet test papers treated with 0.4% coumaphos. This is approximately a LD25 dosage for each strain. For more details see: Insect Molecular Biology (2007) 16(1), 49-60.
Growth protocol
For more details see: Insect Molecular Biology (2007) 16(1), 49-60.
Extracted molecule
total RNA
Extraction protocol
Total RNA was prepared using the Totally RNA kit (Ambion, Inc., Austin, TX, USA), including aaLiCL precipitation step, according to the kit protocol. SAGE library was synthesized using the I-SAGE kit (Invitrogen, Carlsbad, CA, USA). For more details see: Insect Molecular Biology (2007) 16(1), 49-60.
Description
For more details see: Insect Molecular Biology (2007) 16(1), 49-60.
Data processing
Sequence data was processed to extract 14 bp tags using a pipeline of Perl scripts. Duplicate ditags, ditags not of length 28-38 bp, and linker sequences were removed. For more details see: Insect Molecular Biology (2007) 16(1), 49-60.
