|
| Status |
Public on Jan 12, 2010 |
| Title |
male wild type, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
1 day old wilf type male
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Gender: male
Genotype: F1 generation, by crossing virgin Canton S wild type females with Oregon R wild type males
Age: 1 day old
|
| Treatment protocol |
Adult flies were collected and aged to 1 day old in vials containing 4 ml food
|
| Growth protocol |
Stocks were maintained in standard oatmeal and molasses medium containing 1.5% (w/v) sucrose (Merck), 3% glucose (Sigma), 3.5% Instant Dry Baker’s Yeast (European), 1.5% Maize flour (Oriola), 1% Wheat germ (Oriola), 1% Soya flour (Oriola), 1% agar (Oriola) and 3% Lyle’s black treacle (Tate & Lyle, UK), to which was added 0.1% Nipagin M (Sigma) and 0.5% (v/v) propionic acid (JT Baker).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. Afterwars, RNA was cleaned using the RNeasy® MinElute™ Cleanup kit (Qiagen). RNA concentration and purity were checked spectrophotometrically and RNA quality was verified electrophoretically. RNA was stored at –20 ºC in RNase-free water.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA using kits provied by Quiagen
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45ºC on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 High-Resolution.
|
| Description |
male wild type, biological rep3
|
| Data processing |
The data were analyzed with GeneChip Operating Software (GCOS) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jan 14, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Daniel Jose Moreno Fernandez-Ayala |
| E-mail(s) |
[email protected]
|
| Organization name |
Universidad Pablo de Olavide
|
| Lab |
CABD/CSIC-UPO
|
| Street address |
Carretera de Utrera, Km. 1
|
| City |
Sevilla |
| ZIP/Postal code |
41013 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE10169 |
Gene expression in a Drosophila model of mitochondrial disease |
|
| Relations |
| Reanalyzed by |
GSE119084 |
