|
| Status |
Public on Jan 26, 2018 |
| Title |
SJRHB012_Z |
| Sample type |
RNA |
| |
|
| Source name |
Human FN-RMS PDX sample
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: FN-RMS xenograft
|
| Treatment protocol |
shRNA cells treated with 50ng/mL doxycycline.
|
| Growth protocol |
Cells grown in 10% FBS, DMEM at 37°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy extraction of total RNA according to manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
100ng of total RNA were labeled with Cy3 according to Agilent's labeling protocol.
|
| |
|
| Hybridization protocol |
Microarray hybridization was performed at 55°C for 20 hours in Agilent's microarray hybridization chambers, followed by wash procedures according to the manufacturer’s recommended protocols.
|
| Scan protocol |
Microarray was scanned in an Agilent scanner at 5μm resolution, and the array data was extracted with Agilent feature extraction software using the miRNA_105_Jan09 protocol.
|
| Description |
MEH005
Gene expression data from Patient-derived xenograft.
|
| Data processing |
Agilent Feature Extraction Software (version 10.5.1.1) was used, followed by a log transformation, percentile normalization across probes, and scaling through the samples, prior to actual analysis.
|
| |
|
| Submission date |
Apr 09, 2017 |
| Last update date |
Jan 27, 2018 |
| Contact name |
Yong-Dong Wang |
| E-mail(s) |
[email protected]
|
| Phone |
9015954224
|
| Organization name |
St. Jude Children's Research Hospital
|
| Street address |
262 Danny Thomas Pl, MS1135
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38134 |
| Country |
USA |
| |
|
| Platform ID |
GPL19730 |
| Series (1) |
| GSE97553 |
PAX3-FOXO1 regulated microRNAs contribute to the pathogenesis of alveolar rhabdomyosarcoma |
|
