|
| Status |
Public on Mar 16, 2008 |
| Title |
Human induced pluripotent stem cell clone 1-8 cultured in mTeSR1 on Matrigel after freeze-thaw treatment |
| Sample type |
RNA |
| |
|
| Source name |
human induced pluripotent stem cells from fibroblast (5F0438) cultured in mTeSR1 on Matrigel
|
| Organism |
Homo sapiens |
| Characteristics |
human embryonic stem cells like pluripotent stem cells (46XY)
|
| Biomaterial provider |
fibroblast from Lonza
|
| Treatment protocol |
preparation at total passage 29 (183 day culture after 4 factor retroviral gene transfer),
at passage 8 via the second freezing & thawing treatment after 5 passages via the first freezing & thawing treatment after 16 passages following gene transfer
|
| Growth protocol |
cultured in mTeSR1 on Matrigel
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy
|
| Label |
biotin
|
| Label protocol |
Biotin-labelled cRNA was reverse transcribed from 1 µg of total RNA according to Affymetrix technical protocols.
|
| |
|
| Hybridization protocol |
Fifteen micrograms of cRNA was fragmented and hybridized to Affymetrix U133 plus 2 GeneChip arrays at 45C for 16 h and then washed and stained using Affimetrix Fluidics.
|
| Scan protocol |
The arrays were scanned using an Affimetrix GCS3000 scanner.
|
| Description |
Gene expression data from human induced pluripiotent stem cell culture in mTeSR on Matrigel
The same RNA sample was also employed for Agilent array analysis.
|
| Data processing |
The images obtained were processed to data using the GCOS software. Data were analysed with the GeneSpring 7.3.1. software
|
| |
|
| Submission date |
Jan 17, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Tetsuya Ishikawa |
| E-mail(s) |
[email protected]
|
| Phone |
+81-3-3542-2511
|
| Fax |
+81-3-5565-0727
|
| Organization name |
Japanease National Cancer Center
|
| Department |
Research Institute
|
| Lab |
Section for Studies on Metastasis
|
| Street address |
1-1, Tsukiji 5-chome
|
| City |
Chuo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE9709 |
Human induced pluripotent stem (iPS) cells from neonatal skin derived cells |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
