|
| Status |
Public on Mar 16, 2008 |
| Title |
Human induced pluripotent stem cell clone 3-2 cultured in mTeSR1 on Matrigel |
| Sample type |
RNA |
| |
|
| Source name |
human induced pluripotent stem cells from fibroblast (5F0438) cultured in mTeSR1 on Matrigel
|
| Organism |
Homo sapiens |
| Characteristics |
human embryonic stem cells like pluripotent stem cells (46XY)
|
| Biomaterial provider |
fibroblast from Lonza
|
| Treatment protocol |
preparation at passage 7 (80 days after 4 factor retroviral gene transfer)
|
| Growth protocol |
cultured in mTeSR1 on Matrigel
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy
|
| Label |
Cy3
|
| Label protocol |
cRNA synthesis using Agilent low RNA input linear amplication kit and cyanine 3-CTP
|
| |
|
| Hybridization protocol |
Gene Expression Hybridization Kit
|
| Scan protocol |
Agilent microarray scanner
|
| Description |
RNA sample of Agilent array is the same as that of Affymetrix array (GSM257523).
|
| Data processing |
Agilent Feature Extraction software (v.9.5.3); gProcessedSignal; processed Cy3 Signal
|
| |
|
| Submission date |
Jan 17, 2008 |
| Last update date |
Mar 16, 2008 |
| Contact name |
Tetsuya Ishikawa |
| E-mail(s) |
[email protected]
|
| Phone |
+81-3-3542-2511
|
| Fax |
+81-3-5565-0727
|
| Organization name |
Japanease National Cancer Center
|
| Department |
Research Institute
|
| Lab |
Section for Studies on Metastasis
|
| Street address |
1-1, Tsukiji 5-chome
|
| City |
Chuo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1708 |
| Series (1) |
| GSE9709 |
Human induced pluripotent stem (iPS) cells from neonatal skin derived cells |
|
