|
| Status |
Public on Apr 18, 2017 |
| Title |
HM_NSsiRNA |
| Sample type |
RNA |
| |
|
| Source name |
HM cells transfected with NS siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line source: HEYA8
cell type: highly metastatic (HM) ovarian cancer cells
transfected with: non-specific (NS) siRNA
|
| Treatment protocol |
Transfection of NS or β-catenin targeting siRNA were performed using siLectfect (Bio-Rad) for 48 h according to manufacturer's protocol. The siRNA were purchased from Dharmacon.
|
| Growth protocol |
HEYA8 HM cells were cultured in RPMI 1640 medium supplemented with 5% fetal bovine serum and 1% penicillin and streptomycin at 37C in a humidified atmosphere of 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
RNA were biotinylated using the FlashTag Biotin HSR RNA Labelling kit (Affymetrix) with 800ng total RNA as input.
|
| |
|
| Hybridization protocol |
Biotinylated RNA were hybridized on GeneChip miRNA 4.0 Array according to the instructions of the FlashTag Biotin HSR RNA Labelling kit (Affymetrix) . GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000 7G.
|
| Description |
NS-siRNA
|
| Data processing |
The data were processed with Expression Console using the RMA + DABG (with quantile normalization) algorithm.
affymetrix-algorithm-name = rma : dabg
affymetrix-algorithm-version = 1.0
program-name = Expression Console
program-version = 1.4.1.46
|
| |
|
| Submission date |
Apr 17, 2017 |
| Last update date |
Apr 18, 2017 |
| Contact name |
Kit Yan To |
| Organization name |
The University of Hong Kong
|
| Department |
School of Biological Sciences
|
| Street address |
Pokfulam Road
|
| City |
Hong Kong |
| ZIP/Postal code |
N/A |
| Country |
Hong Kong |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE97864 |
Effects of β-catenin knockdown on microRNAome in highly metastatic ovarian cancer cells |
|
