|
| Status |
Public on Apr 19, 2017 |
| Title |
Human non-trisomy embryo 3 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Human reference DNA
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: SureRef reference DNA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Whole Genome Amplification performed with Sureplex following manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Labelling was done following standard 24sure v3 protocol. In brief, samples were mixed with 5µl of random primers and denaturated for 5min at 94°C. Afterwards, samples were mixed with 12µl of Cy3/Cy5 labelling master mix and incubated at 37°C for 4 hours.
|
| |
|
| Channel 2 |
| Source name |
Trophectoderm human embryo cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Human embryo trophectoderm cells biopsied at day 5 by conventional method
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Whole Genome Amplification performed with Sureplex following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Labelling was done following standard 24sure v3 protocol. In brief, samples were mixed with 5µl of random primers and denaturated for 5min at 94°C. Afterwards, samples were mixed with 12µl of Cy3/Cy5 labelling master mix and incubated at 37°C for 4 hours.
|
| |
|
| |
| Hybridization protocol |
Samples were dissolved in hybridization mix at 75°C for 10 minutes, and incubated overnight at 47°C on the arrays in closed incubation chambers with 50% formamide. The slides were washed 10min in 2xSSC/0.05% Tween20, 10 min 1xSSC, 5min 0.1%SSC at 60°C and 1min in 0.1%SSC at RT
|
| Scan protocol |
Scanned on an Innopsys InnoScan 710 laser scan.
|
| Description |
Biological replicate 3 of 9. WGA from biopsied cells obtained from day 5 human embryos, classified by PCR as an embryo lacking trisomies in chromosomes 18 and 21.
Sample assayed against both female and male references ('combined' file represents combined result of both tests)
|
| Data processing |
Images were imported as .tiff and analyzed using BlueFuse Multi 4.3(25799) Software.
|
| |
|
| Submission date |
Apr 17, 2017 |
| Last update date |
Apr 19, 2017 |
| Contact name |
Esther Lopez-Bayghen |
| E-mail(s) |
[email protected]
|
| Phone |
5557473800
|
| Organization name |
Cinvestav-IPN
|
| Department |
Toxicología
|
| Lab |
Laboratory 19
|
| Street address |
Av. IPN 2508 San Pedro Zac
|
| City |
México |
| State/province |
D.F. |
| ZIP/Postal code |
07360 |
| Country |
Mexico |
| |
|
| Platform ID |
GPL17760 |
| Series (1) |
| GSE97903 |
Trisomy detection by PCR and comparative genomic hybridization |
|
