|
| Status |
Public on Apr 19, 2017 |
| Title |
Human trisomy 21 embryo 4 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Human reference DNA
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: SureRef reference DNA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Whole Genome Amplification performed with Sureplex following manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Labelling was done following standard 24sure v3 protocol. In brief, samples were mixed with 5µl of random primers and denaturated for 5min at 94°C. Afterwards, samples were mixed with 12µl of Cy3/Cy5 labelling master mix and incubated at 37°C for 4 hours.
|
| |
|
| Channel 2 |
| Source name |
Trophectoderm human embryo cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Human embryo trophectoderm cells biopsied at day 5 by conventional method
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Whole Genome Amplification performed with Sureplex following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Labelling was done following standard 24sure v3 protocol. In brief, samples were mixed with 5µl of random primers and denaturated for 5min at 94°C. Afterwards, samples were mixed with 12µl of Cy3/Cy5 labelling master mix and incubated at 37°C for 4 hours.
|
| |
|
| |
| Hybridization protocol |
Samples were dissolved in hybridization mix at 75°C for 10 minutes, and incubated overnight at 47°C on the arrays in closed incubation chambers with 50% formamide. The slides were washed 10min in 2xSSC/0.05% Tween20, 10 min 1xSSC, 5min 0.1%SSC at 60°C and 1min in 0.1%SSC at RT
|
| Scan protocol |
Scanned on an Innopsys InnoScan 710 laser scan.
|
| Description |
Biological replicate 4 of 7. WGA from biopsied cells obtained from day 5 human embryos, classified by PCR as an embryo with a trisomy in chromosome 21
Sample assayed against both female and male references ('combined' file represents combined result of both tests)
|
| Data processing |
Images were imported as .tiff and analyzed using BlueFuse Multi 4.3(25799) Software.
|
| |
|
| Submission date |
Apr 17, 2017 |
| Last update date |
Apr 19, 2017 |
| Contact name |
Esther Lopez-Bayghen |
| E-mail(s) |
[email protected]
|
| Phone |
5557473800
|
| Organization name |
Cinvestav-IPN
|
| Department |
Toxicología
|
| Lab |
Laboratory 19
|
| Street address |
Av. IPN 2508 San Pedro Zac
|
| City |
México |
| State/province |
D.F. |
| ZIP/Postal code |
07360 |
| Country |
Mexico |
| |
|
| Platform ID |
GPL17760 |
| Series (1) |
| GSE97903 |
Trisomy detection by PCR and comparative genomic hybridization |
|
