|
| Status |
Public on Apr 24, 2018 |
| Title |
WT pol2 ChIP 45' |
| Sample type |
SRA |
| |
|
| Source name |
whole organism
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: YLL237
genotype: W303-1, RAD5+, bar1delta, POL2-13MYC::KANMX4R, 3XFlag-POL1
phase: release from G1 block
time point: 45'
chip antibody: 9E10 from culture supernatant; Custom mouse monoclonal anti-myc
treatment: alpha factor
|
| Treatment protocol |
Synchronisation as in Mattarocci et al. 2014 (PMID: 24685139 DOI: 10.1016/j.celrep.2014.03.010)
|
| Growth protocol |
Growth conditions as in Mattarocci et al. 2014 (PMID: 24685139 DOI: 10.1016/j.celrep.2014.03.010)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were homogenized by bead beating and treated with proteinase K, followed by Phenol-Chloroform extraction.
Library preparation was carried out at the genomics platform of the University of Geneva using the Illumina DNA-seq nano kit
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
genotype: W303-1, RAD5+, bar1∆, POL2-13MYC::KANMX4R, 3XFlag-POL1
|
| Data processing |
Raw reads were aligned to the sacCer3 genome assembly using Bowtie2 with options "--end-to-end --sensitive -k 20"
Data were filtered to retain only the mapped reads with at most 5 hits in the reference.
reads were extended by read length (50bp)
for ChEC-seq: ChrM was ommited from reference genome
Genome_build: Saccer3
Supplementary_files_format_and_content: .bw
|
| |
|
| Submission date |
Apr 19, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Lukas Hafner |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Geneva
|
| Department |
Molecular Biology
|
| Lab |
Shore
|
| Street address |
30 quai Ernest-Ansermet
|
| City |
Geneva |
| State/province |
Geneva |
| ZIP/Postal code |
1211 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL17342 |
| Series (1) |
| GSE97953 |
Rif1 Binding and Control of Chromosome-Internal DNA Replication Origins Is Limited by Telomere sequestration |
|
| Relations |
| BioSample |
SAMN06765798 |
| SRA |
SRX2745395 |
