|
| Status |
Public on Nov 05, 2021 |
| Title |
RGS1, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Cord blood CD34+ cells, RGS1
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Cord blood CD34+ cells
expression: RGS1
|
| Treatment protocol |
GFP control, RGS1, RGS13 and RGS16 transduction.
|
| Growth protocol |
Cord blood CD34+ cells were grown in Stemline II medium supplemented with TPO, SCF and Flt-3L, cells were subjected to 2 rounds of lentiviral transduction at MOI 4-8. Cells were harvested for gene expression profiling 24 hours after the last transduction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the Trizol Reagent (Invitrogen, Carlsbad, CA) and RNeasy micro kit (Qiagen, Valencia, CA) following the manufacturer's recommendations. The RNA quality and integrity were analyzed with Nanodrop ND 1000 spectrophotometer (Rockland, DE) and Bioanalyzer (Agilent, Cheshire, UK).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (Two-Cycle cDNA Synthesis Kit and IVT labeling kit).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome Gene v2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400 (EukGE-Ws2v4).
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
RGS1-3
Gene expression data from cord blood CD34+ cells.
|
| Data processing |
The digital data retrieved from the CEL files were processed by Partek Genomic Suite version 6.6 using modified RMA-algorithm.
|
| |
|
| Submission date |
May 02, 2017 |
| Last update date |
Nov 05, 2021 |
| Contact name |
Kathy YY Chan |
| E-mail(s) |
[email protected]
|
| Phone |
852-2632-2858
|
| Organization name |
The Chinese University of Hong Kong
|
| Department |
Paediatrics
|
| Street address |
Rm84041, 6/F, Clinical Science Building, Prince of Wales Hospital, Shatin, N.T.
|
| City |
Hong Kong |
| ZIP/Postal code |
nil |
| Country |
Hong Kong |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE98453 |
R4 RGS subfamily proteins regulates human hematopoietic stem cell engraftment |
|
