|
| Status |
Public on Nov 03, 2017 |
| Title |
WT_2weeks_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
B6_ treated-2weeks
|
| Organism |
Mus musculus |
| Characteristics |
tissue: glomerular extracts
strain: C57Bl/6
genotype/variation: wildtype
timepoint: 2 weeks
|
| Treatment protocol |
Mice were injected i.p. with 8ul/g nephrotoxic serum from sheep to induce anti-GBM nephritis before kidney harvest.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from 600µL glomerular extracts with the Direct-zol RNA Mini-prep kit (Zymo Research) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Fragmented biotin-labeled cDNA (from 10ng RNA) was synthesized using the Affymetrix WT Pico kit.
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Mouse Transcriptome Array 1.0 (MTA 1.0) (Affymetrix) and scanned on an Affymetrix Gene Chip Scanner 3000 using Command Console software.
|
| Scan protocol |
Scanning was performed according to manufacturer's instructions (Affymetrix).
|
| Data processing |
Quality control of the experiment was performed by Expression Console software (Affymetrix) for normalization and background correction. Data analysis and statistical evaluations were performed using Transcriptome Analysis Console (Affymetrix). Significance in gene expression determined by ANOVA p<0.05.
|
| |
|
| Submission date |
May 08, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Roberto Caricchio |
| E-mail(s) |
[email protected]
|
| Organization name |
Lewis Katz School of Medicine
|
| Department |
Medicine/Rheumatology
|
| Lab |
Caricchio's Lab
|
| Street address |
3322 N. Broad Street
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19140 |
| Country |
USA |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE98626 |
Estrogen signaling through Estrogen Receptor ⍺ alters lipid metabolism and promotes glomerulonephritis |
|
