|
| Status |
Public on Oct 10, 2008 |
| Title |
_9_cy5_Anaerobic_Stationary_Phase |
| Sample type |
RNA |
| |
|
| Source name |
_9_cy5_Anaerobic_Stationary_Phase
|
| Organism |
Zymomonas mobilis |
| Characteristics |
Stationary_Phase__26h__F4_Anaerobic
|
| Treatment protocol |
Anaerobic
|
| Growth protocol |
Z. mobilis ZM4 was obtained from the American Type Culture Collection and cultured in RM medium at 30 C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples from aerobic and anaerobic fermentors were harvested by centrifugation and the TRIzol reagent (Invitrogen) was used to extract total cellular RNA. Each total RNA preparation was treated with RNase-free DNase I (Ambion, Austin, TX) to digest residua
|
| Label |
cy5
|
| Label protocol |
The purified RNA from each sample was used as the template to generate cDNA copies labeled with either Cy3-dUTP or Cy5-dUTP.
|
| |
|
| Hybridization protocol |
The two separately labeled cDNA pools to be compared were mixed together in a hybridization solution containing 50% formamide, applied to microarrays and incubated overnight at 50 C. Microarrays were washed using buffers of increasing stringency accordin
|
| Scan protocol |
The sample was scanned with a ScanArray 5000 Microarray Analysis System PerkinElmer Life Sciences Inc, Boston, MA and images were quantified using Microarray Imager software from Combimatrix.
|
| Description |
Stationary_Phase__26h__F4_Anaerobic
|
| Data processing |
Microarray data was log 2 transformed and normalized using the standard normalization routine as implemented in JMP Genomics 3.0 (SAS Institute Inc., Cary, NC).
|
| |
|
| Submission date |
Jan 28, 2008 |
| Last update date |
Jul 03, 2008 |
| Contact name |
Stan Martin |
| Organization name |
Science Apps, L3C
|
| Street address |
1404 Crete Dr.
|
| City |
Raleigh |
| State/province |
NC |
| ZIP/Postal code |
27606 |
| Country |
USA |
| |
|
| Platform ID |
GPL6431 |
| Series (1) |
| GSE10302 |
Zymomonas mobilis oxygen stress responses |
|
