|
| Status |
Public on May 16, 2017 |
| Title |
AE104 with 100 µM ZnCl2, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
cells of C. metallidurans
|
| Organism |
Cupriavidus metallidurans CH34 |
| Characteristics |
genotype: AE104
|
| Treatment protocol |
ZnCl2 was added at a cell turbitity of 100 Klett for 10 min
|
| Growth protocol |
C. metallidurans strains were grown in Mineral salts medium + 0.2 % Na-gluconate to a cell turbidity of 100 Klett, ZnCl2 was added and after 10 min cells were harvest by centrifugation for 1' at RT in 2.0 ml Tubes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plus Mini Kit (Qiagen) was used according to the manufacturers manual including on-column DNase digestion. PCR were done to check for DNA contaminations, only RNA was used that did not generate products in the PCR reaction. RNA quality was checked on formamide gels and measured as RIN on Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany).
|
| Label |
Cy3
|
| Label protocol |
Labeling was done by the service provider IMGM Laboratories GmbH (Martinsried)
|
| |
|
| Hybridization protocol |
Hybridisation was done by the service provider IMGM Laboratories GmbH (Martinsried)
|
| Scan protocol |
Agilent DNA microarray Scanner were used by the service provider IMGM Laboratories GmbH (Martinsried)
|
| Data processing |
The mean intensity of the pixels of the surrounding area was subtracted from the mean density of the pixels of the spots to give the signal strength
|
| |
|
| Submission date |
May 15, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Cornelia Große |
| E-mail(s) |
[email protected]
|
| Organization name |
Martin-Luther-University Halle-Wittenberg
|
| Department |
Faculty of Biology/Microbiology
|
| Street address |
Kurt-Mothes-Str.3
|
| City |
Halle/Saale |
| ZIP/Postal code |
06120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16115 |
| Series (1) |
| GSE98926 |
Influence of AE104(∆zur) mutant of Cupriavidus metallidurans on Zn stress |
|
