|
| Status |
Public on Feb 10, 2021 |
| Title |
Macrophages, IFNgamma stimulation, donor 1 |
| Sample type |
RNA |
| |
|
| Source name |
Buffy coat, donor 1
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: PBMC derived monocytes
donornr: 1
treatment: IFN
titanium: ctrl
|
| Treatment protocol |
Macrophages were cultured on cell culture plastic or on polished titanium discs. Additionally they were stimulated at day 0 either with IL-4 (10ng/ml) or with IFNgamma (100ng/ml) or with M2Cocktail(10ng/ml M-CSF + 3ng/ml IL-4 + 10ng/ml IL-10 + 10ng/ml TGFbeta) or left untreated as control.
|
| Growth protocol |
Macrophages were cultured in Macrophage Serum Free Medium at a concentration of 2x10^6 cells/ml, supplemented with 1ng/ml M-CSF, 10^-8M Dexamethasone for 6 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using E.Z.N.A. total RNA Kit I according to manufacturer´s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
Gene expression data from human macrophages stimulated with IFNgamma and cultured for 6 days on cell culture plastic
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 7, from SAS. Gene expression profiling was performed using arrays of human Hugene-1_0-type from Affymetrix. A Custom CDF Version 19 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
|
| |
|
| Submission date |
May 30, 2017 |
| Last update date |
Feb 10, 2021 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL20171 |
| Series (2) |
| GSE99446 |
Gene expression data of macrophages cultured on polished titanium discs |
| GSE99448 |
Titanium induces pro-inflammatory and tissue remodeling responses in human macrophages |
|
