|
| Status |
Public on Oct 20, 2020 |
| Title |
Macrophages, without stimulation + EtOH 70%, donor 858-3 |
| Sample type |
RNA |
| |
|
| Source name |
Buffy coat, donor 858-3
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: PBMC derived monocytes
sample: BC 858-3
stimulation: Ns
plant extract: Ctrl
|
| Treatment protocol |
Macrophages were stimulated at day 0 either with IFNgamma (100ng/ml) or left untreated as control and cultured with extracts of either S. roemeri (1:500) or S. sendtneri (1:500) or with 70% EtOH (1:500) as control.
|
| Growth protocol |
Macrophages were cultured in Macrophage Serum Free Medium at a concentration of 1x10^6 cells/ml, supplemented with 5ng/ml M-CSF, 10^-8M Dexamethasone for 6 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using E.Z.N.A. total RNA Kit I according to manufacturer´s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
Gene expression data from human unstimulated macrophages cultured for 6 days with 70% EtOH
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 6, from SAS. Gene expression profiling was performed using arrays of human Hugene-2_0-type from Affymetrix. A Custom CDF Version 19 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
|
| |
|
| Submission date |
May 30, 2017 |
| Last update date |
Oct 20, 2020 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL23526 |
| Series (1) |
| GSE99447 |
The effect of plant extracts on human macrophage activation |
|
