|
| Status |
Public on Jun 05, 2021 |
| Title |
WT (wild type) _Day 0,biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
WT (wild type) _Day 0,biological rep1
|
| Organism |
Mycobacterium tuberculosis H37Rv |
| Characteristics |
genotype: WT
treatment: None
|
| Treatment protocol |
Cultures were incubated for 7 days with /without the addition of GSK-X9.
|
| Growth protocol |
Early stationary phase cultures of the WT and Δrel strains were resuspended in PBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNAs were prepared with the Affymetrix FlashTag Biotin HSR 3' IVT Express Kit per manufacturer's instructions.
|
| |
|
| Hybridization protocol |
16hrs at 45° C with rotation (60rpm) as described by Affymetrix in their GeneChip Expression Analysis Technical Manual.
|
| Scan protocol |
Using Affymetrix’ GeneChip Scanner 3000 7G and default parameters described by the manufacturer in their GeneChip Expression Analysis Technical Manual.
|
| Data processing |
Raw CEL file data were extracted, RMA normalized and transformed to log2 notation with the Partek Genome Suite v6.6 platform.
|
| |
|
| Submission date |
Jun 06, 2017 |
| Last update date |
Jun 05, 2021 |
| Contact name |
Noton K Dutta |
| E-mail(s) |
[email protected]
|
| Organization name |
Johns Hopkins University
|
| Department |
Medicine
|
| Lab |
Center for Tuberculosis Research
|
| Street address |
1550 Orleans Street, Room 110
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21287 |
| Country |
USA |
| |
|
| Platform ID |
GPL4519 |
| Series (1) |
| GSE99710 |
Validation of RelMtb as a target for Mycobacterium tuberculosis persisters |
|
