|
| Status |
Public on Jun 05, 2018 |
| Title |
ED2_2 |
| Sample type |
SRA |
| |
|
| Source name |
B. germanica embryo day 2
|
| Organism |
Blattella germanica |
| Characteristics |
developmental stage: Embryo day 2
tissue: Whole body
|
| Growth protocol |
Blattella germanica specimens used for the whole transcriptome analysis were obtained from a population reared at the Institut de Biologia Evolutiva (CSIC-UPF). Conditions were 70% humidity at 30 ºC and a commercial dog food diet.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
GenElute ™ Mammalian Total RNA Miniprep Kit.
NEBNext® mRNA Library Prep master mix for Illumina®.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina MiSeq |
| |
|
| Description |
mRNA
Processed data file: mRNA_Counts_table_anotv2.txt
|
| Data processing |
Quality control with FastQC.
Read filtering and trimming with Trimmomatic.
Reads mapping with Bowtie.
Gene counts with FeatureCounts.
Genome_build: https://www.hgsc.bcm.edu/arthropods/german-cockroach-genome-project
Supplementary_files_format_and_content: mRNA_Counts_table_anotv2.txt: Tab-delimited text file includes counts.
|
| |
|
| Submission date |
Jun 07, 2017 |
| Last update date |
Jun 05, 2018 |
| Contact name |
Guillem Ylla |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute of Evolutionary Biology
|
| Lab |
Belles & Piulachs Labs
|
| Street address |
Passeig Marítim de la Barceloneta, 37
|
| City |
Barcelona |
| State/province |
Catalonia |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL22010 |
| Series (1) |
| GSE99785 |
Comparative transcriptomics in two extreme neopterans reveal general trends in the evolution of modern insects |
|
| Relations |
| BioSample |
SAMN07203208 |
| SRA |
SRX2892914 |
