|
| Status |
Public on Jun 10, 2017 |
| Title |
WT DX5- NK2 |
| Sample type |
RNA |
| |
|
| Source name |
C57BL/6 mice liver
|
| Organism |
Mus |
| Characteristics |
strain: C57BL/6
genotype: WT
tissue: liver
cell type: DX5- NK cell
gender: female
old: 6-8 week
|
| Treatment protocol |
WT and dnTGFbRII mice liver DX5- NK cells were sorted for RNA extraction and hybridization on Affymetrix microarrays.
|
| Growth protocol |
WT and dnTGFbRII mice liver DX5- NK cells were sorted
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The fragmented cDNA is labeled by terminal deoxynucleotidyl transferase (TdT) using the Affymetrix proprietary DNA Labeling Reagent that is covalently linked to biotin. 5.5 μg of single- stranded cDNA is required for fragmentation and labeling.
|
| |
|
| Hybridization protocol |
Array hybridization and washing was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in a Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and a Fluidics Station 450 (Cat#00-0079, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
|
| Scan protocol |
Arrays were scanned by Affymetrix GeneChip® Scanner 3000 (Cat#00-00213, Affymetrix, Santa Clara, CA, US).
|
| Description |
WT DX5- 2
|
| Data processing |
Command Console Software (Affymetrix, Santa Clara, CA, US) was used to control the scanner and summarize probe cell intensity data (CEL file generation) with default settings. Then raw data were RMA-normalized by Expression Console.
|
| |
|
| Submission date |
Jun 09, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Zhe-Xiong Lian |
| Organization name |
University of Science and Technology of China
|
| Department |
School of Life Sciences and Institution of Immunology
|
| Lab |
Liver Immunology Lab
|
| Street address |
Huangshan Road 443, Western Campus of USTC
|
| City |
Hefei |
| State/province |
Anhui |
| ZIP/Postal code |
230027 |
| Country |
China |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE99809 |
Expression data from WT liver DX5- NK cells and dnTGFbRII liver DX5- NK cells |
|
