|
| Status |
Public on Oct 01, 2008 |
| Title |
HL-1 cells with rapid electric stimulation for 24 hr at 300 bpm (exp 2) |
| Sample type |
RNA |
| |
|
| Source name |
HL-1 cells with rapid electric stimulation for 24 hr at 300 bpm, experiment 2
|
| Organism |
Mus musculus |
| Characteristics |
HL-1 cells with rapid electric stimulation for 24 hr at 300 bpm
|
| Treatment protocol |
HL-1 cells were cultured in supplemented Claycomb Media (Sigma-Aldrich) to confluence then electrically stimulated for 24 hr at 300 bpm (approximately 1.5-2 times greater than control).
|
| Growth protocol |
HL-1 cells were cultured in supplemented Claycomb Medium (Sigma-Aldrich) as previously described. Culture dishes were pre-plated with a sterilized, gelatin/fibronectin-coated coverslip, then seeded with HL-1 cells, typically reaching at least 90% confluency at 96 hr.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were extracted from control and stimulated cells using the RNAeasy Mini Kit (Animal Cell Protocol, Qiagen). To ensure rigorous quality control, all samples were analyzed by the Vanderbilt Microarray Shared Resource for concentration, purity, and integrity (degradation) using spectrophotometry (NanoDrop ND-1000 UV-Vis Spectrophotometer) and a microfluidic assay (Agilent 2100 Bioanalyzer, Agilent Technologies).
|
| Label |
biotin
|
| Label protocol |
biotinylated cRNA, as well as subsequent steps in completion of the hybridization following the Affymetrix One-Cycle Target Labeling protocol
|
| |
|
| Hybridization protocol |
Affymetrix protocol, Vanderbilt Microarray Shared Resource completed this step accordingly
|
| Scan protocol |
Affymetrix protocol, Vanderbilt Microarray Shared Resource completed this step accordingly
|
| Description |
HL-1 cells with rapid electric stimulation for 24 hr at 300 bpm
|
| Data processing |
Vanderbilt Microarray Shared Resource completed this step accordingly. CEL files were normalized by RMA method
|
| |
|
| Submission date |
Feb 21, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
yajun yi |
| E-mail(s) |
[email protected]
|
| Organization name |
vanderbilt univ
|
| Street address |
2215 Garland Ave
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE10598 |
Transcriptional profile of rapidly stimulated atrial myocytes: Conservation with human atrial fibrillation |
|
| Relations |
| Reanalyzed by |
GSE119085 |
