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Status
Public on Sep 28, 2004
Title
day 6 5-FU HSC 1
Sample type
RNA
Source name
Sca+ SP (side population) bone marrow cells
Organism
Mus musculus Extracted molecule
total RNA
Description
HSC (Sca+ SP) were isolated from 8-12 week C57B6 mice at various time points after treatment with 5-Fluorouracil. RNA was isolated from 50,000-100,000 FACS sorted cells and subjected to two round of T7 based linear amplification using Ambion's Message Amp kit. Two replicates from each time point were analyzed.
Submission date
Jul 12, 2004
Last update date
May 27, 2005
Contact name
Margaret A Goodell
E-mail(s)
[email protected] Phone
713-798-1265
Organization name
Baylor College of Medicine
Department
Center for Cell and Gene Therapy
Street address
One Baylor Plaza, N1030
City
Houston
State/province
TX
ZIP/Postal code
77030
Country
USA
Platform ID
GPL81 Series (1)
Data table header descriptions ID_REF
VALUE 'signal' a measure of the abundance of a transcript determined by MAS 5.0
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC) (MAS 5.0)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call (MAS 5.0)
GC-RMA VALUE normalized expression measure using GC-RMA procedure (www.bioconductor.org)
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE GC-RMA VALUE 100001_at
7.7
A
0.732537
1.198
100002_at
20.4
A
0.621816
1.364
100003_at
83.6
A
0.127645
1.177
100004_at
556.4
P
0.000562
3.019
100005_at
255.8
P
0.006532
1.802
100006_at
13.5
A
0.397994
1.227
100007_at
444.7
P
0.021866
2.428
100009_r_at
28.3
A
0.107301
1.242
100010_at
255.7
A
0.060419
1.908
100011_at
1388.9
P
0.000266
7.908
100012_at
5280.1
P
0.000322
10.026
100013_at
548.5
P
0.014937
5.59
100014_at
393.6
P
0.000219
4.855
100015_at
330.9
M
0.043968
2.107
100016_at
97.6
P
0.009985
1.679
100017_at
108
A
0.07383
1.523
100018_at
276.6
A
0.284747
1.943
100019_at
57.3
A
0.64131
1.6
100020_at
395.5
P
0.011447
3.266
100021_at
11.9
A
0.67917
1.257
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