|
| Status |
Public on May 31, 2018 |
| Title |
NO2-CLA2 |
| Sample type |
SRA |
| |
|
| Source name |
hCASMC
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human coronary artery smooth muscle cell
passages: 5-6
agent: NO2-CLA
|
| Treatment protocol |
The cells at 80% confluence were maintained in 0.5% FBS medium for overnight and subsequently treated with NO2-CLA, CLA or vehicle control for 6 hours
|
| Growth protocol |
Human coronary artery smooth muscle cells (hCASMCs) were purchased from PromoCell (Germany) and cultured in Smooth Muscle Cell Growth Medium 2 from same company at 37 °C, 5% CO2 in a standard cell culture incubator
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA from hCASMCs were isolated and purified using RNeasy mini kit (Qiagen) and subjected to on-column RNase-free DNase treatment following the manufacturer’s recommendation (Qiagen)
RNA-seq library was prepared using the EnCore Complete RNA Seq Library System (NuGen, San Carlos, CA)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
cDNA sample from hCASMC cells treated with NO2-CLA, replicate 2
|
| Data processing |
Read quality check using FastQC
Read mapping to reference genome using Cufflinks, FPKM values were generated
Genome_build: GRCh37 (Ensembl)
Supplementary_files_format_and_content: fpkm_gene.txt
|
| |
|
| Submission date |
Jun 30, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Shengdi Li |
| E-mail(s) |
[email protected]
|
| Organization name |
SIBS
|
| Street address |
320 Yueyang Road
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE100676 |
Transcriptome sequencing of human coronary artery smooth muscle cell under treatment of nitrated fatty acid |
|
| Relations |
| BioSample |
SAMN07303248 |
| SRA |
SRX2973181 |
